Composite

Part:BBa_K4044007

Designed by: Kalinichenko Andrei   Group: iGEM21_LMSU   (2021-10-20)
Revision as of 08:18, 21 October 2021 by Alexandr56 (Talk | contribs)

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p119_BphP1-BcLOV4_QPAS1-Gal4 test system with p119_G4-n11_YFP

This is a composite part of BphP1-BcLOV4 construction under pj23119 promoter (BBa_J23119) fused to QPAS1-Gal4 construction forming a whole operon; with YFP gene under p119_G4-n11 promoter (BBa_K4044011). The part is assembled for testing the accuracy and dynamic range of the system with p119_G4-n11 promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 815
    Illegal PstI site found at 1112
    Illegal PstI site found at 1559
    Illegal PstI site found at 2608
    Illegal PstI site found at 2852
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 4282
    Illegal PstI site found at 815
    Illegal PstI site found at 1112
    Illegal PstI site found at 1559
    Illegal PstI site found at 2608
    Illegal PstI site found at 2852
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 301
    Illegal BamHI site found at 1876
    Illegal BamHI site found at 4029
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 815
    Illegal PstI site found at 1112
    Illegal PstI site found at 1559
    Illegal PstI site found at 2608
    Illegal PstI site found at 2852
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 815
    Illegal PstI site found at 1112
    Illegal PstI site found at 1559
    Illegal PstI site found at 2608
    Illegal PstI site found at 2852
    Illegal NgoMIV site found at 457
    Illegal NgoMIV site found at 1208
    Illegal NgoMIV site found at 1705
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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