Part:BBa_K4083012

TetR

tetR gene encodes TetR which is also called Tet repressor proteins.

Usage and Biology

In the natural world, TetR decreases the activity of repressor for the tetracycline resistance gene (tetA) promoter. (tetA) For the RemiDuET project, this part was used in the pRGPDuo2 [2] plasmid to regulate the tetR/tetA promoter.

Reference

[1]UniProt. (n.d.-b). Tetracycline repressor protein class B from transposon Tn10. Retrieved October 21, 2021, from https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=&cad=rja&uact=8&ved=2ahUKEwiLsYy_hNvzAhXisYsKHSTJDFsQFnoECDYQAQ&url=https%3A%2F%2Fwww.uniprot.org%2Funiprot%2FP04483&usg=AOvVaw0_D4Dog1UDqbMVDAZYCq7m

[2] Gauttam, R., Mukhopadhyay, A., & Singer, S. W. (2020). Construction of a novel dual-inducible duet-expression system for gene (over)expression in Pseudomonas putida. Plasmid, 110. https://doi.org/10.1016/j.plasmid.2020.102514

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]