Part:BBa_K3788000

StrepII-Cyt1Aa

BBa_K3788000 sequence is coding for Cyt1Aa, a toxins from Bacillus thuringiensis. This toxin has a strep tag II in N-ter to allow purification of the protein Cyt1Aa and specific detection by Western blot. The gene cyt1Aa was optimised for E. coli K12.

Expected size :

strepII_cyt1Aa gene is 777bp

Strep_Cyt1Aa protein is 28,5 kDa

Mode of action of Cyt toxins:

Cyt (Cytolitic) toxins are produced in pro-toxin form, they will undergo proteolytic cleavage in N-ter and C-ter allowing the release of the active form of Cyt toxins. The mature forms will interact with the membrane lipids of the midgut cells and will insert themselves into the membrane, forming β-barrels. Cyt toxins are different in them domains.

Cyt1Aa would facilitate the binding of Cry11Aa to the BBMV receptor, by the interaction of the loop α8, β4 and loop 2 regions of Cry11Aa at the level of the loop β6 – αE and part of β7 of Cyt1Aa. This interaction allows easy insertion of Cry11Aa into membranes.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]