Coding

Part:BBa_K4083010

Designed by: Arsen Orazbek   Group: iGEM21_NU_Kazakhstan   (2021-10-20)
Revision as of 07:58, 21 October 2021 by Arsenorazbek (Talk | contribs)

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LacI Reverse

This gene encodes the repressor of the lactose operon.

Usage and Biology

This gene is universally used to control the plac or its derivatives as ptac promoters [1]. However, For our main project goal, we reversed the lacI gene according to the pRGPDuo2 plasmid.

Reference

[1]Swerdlow, S. J., & Schaaper, R. M. (2014). Mutagenesis in the lacI gene target of E. coli: Improved analysis for lacId and lacO mutants. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 770, 79–84. https://doi.org/10.1016/j.mrfmmm.2014.09.004

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 59
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
reversed_version