Coding
Part:BBa_K4044001:Design
Designed by: Kalinichenko Andrei Group: iGEM21_LMSU (2021-10-19)
Revision as of 07:57, 21 October 2021 by Alexandr56 (Talk | contribs)
BphP1 (E.coli optimized)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 760
Illegal PstI site found at 1057
Illegal PstI site found at 1504 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 760
Illegal PstI site found at 1057
Illegal PstI site found at 1504 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 246
Illegal BamHI site found at 1821 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 760
Illegal PstI site found at 1057
Illegal PstI site found at 1504 - 25INCOMPATIBLE WITH RFC[25]Unknown
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimization of the nucleotide sequence for efficient gene expression in E.coli was performed using GENEWIZ codon optimization tool.
Source
BphP1 protein sequence was taken from https://www.uniprot.org/uniprot/A0A161I5N6 and codon optimized for E. coli.
Source organism: Rhodopseudomonas palustris