Coding

Part:BBa_K4044001:Design

Designed by: Kalinichenko Andrei   Group: iGEM21_LMSU   (2021-10-19)
Revision as of 07:57, 21 October 2021 by Alexandr56 (Talk | contribs)


BphP1 (E.coli optimized)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 760
    Illegal PstI site found at 1057
    Illegal PstI site found at 1504
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 760
    Illegal PstI site found at 1057
    Illegal PstI site found at 1504
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 246
    Illegal BamHI site found at 1821
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 760
    Illegal PstI site found at 1057
    Illegal PstI site found at 1504
  • 25
    INCOMPATIBLE WITH RFC[25]
    Unknown
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization of the nucleotide sequence for efficient gene expression in E.coli was performed using GENEWIZ codon optimization tool.


Source

BphP1 protein sequence was taken from https://www.uniprot.org/uniprot/A0A161I5N6 and codon optimized for E. coli.

Source organism: Rhodopseudomonas palustris

References