Coding
Part:BBa_K3712011
Designed by: Yifan Li Group: iGEM21_HUST2-China (2021-10-20)
Flexible peptide
USAGE AND BIOLOGY
We use it when connecting ELP with other proteins, because it helps maintain the conformation of the two proteins
RESULTS
After culturing, induction and sterilization, the results of nickel column affinity chromatography show that both of Wild-type BLP-7-27 ELP and Optimized BLP-7-27 ELP elution have single peaks at 80 mM with good symmetry, which preliminarily indicates that the target protein is purified. Then we take the BCA method to determine the concentration (Table1) and SDS-PAGE to verify the existence of product.Flexible peptide linking ELP and BLP-7 works well, but linking ELP and TLR2 antagonist with flexible peptide will cause peptide fragmentation.
Future direction:
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |