Plasmid

Part:BBa_K3765011:Design

Designed by: Dipayan Akhuli   Group: iGEM21_IISc-Bangalore   (2021-10-19)
Revision as of 07:07, 21 October 2021 by Dipayan akhuli (Talk | contribs) (→‎References)

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pETMCN-T7


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 5105
    Illegal SpeI site found at 5030
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 5111
    Illegal SpeI site found at 5030
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4964
    Illegal BamHI site found at 5093
    Illegal XhoI site found at 5075
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 5105
    Illegal SpeI site found at 5030
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 5105
    Illegal SpeI site found at 5030
    Illegal NgoMIV site found at 137
    Illegal NgoMIV site found at 3184
    Illegal NgoMIV site found at 3344
    Illegal NgoMIV site found at 4932
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2263


Design Notes

None.

Source

Synthetic.

References

  1. Palani S, Köster DV, Hatano T, Kamnev A, Kanamaru T, Brooker HR, Hernandez-Fernaud JR, Jones AME, Millar JBA, Mulvihill DP, Balasubramanian MK. Phosphoregulation of tropomyosin is crucial for actin cable turnover and division site placement. J Cell Biol. 2019 Nov 4;218(11):3548-3559. doi: 10.1083/jcb.201809089.