Part:BBa_K3739056

J23100-B0030-LamB1-linker-LamB2-his-B0010

This is another anchored proteins onto membranes through LamB and use his tag to comformation. We use BBa_K3739056 and his tag to verify LamB's function which can anchor proteins onto membranes.

Biology

LamB is an anchor protein from Vibrio Species, which can anchor its passenger protein to the cell membrane. It has been widely used in cell-surface display. The linker is a meaningless short peptide that we use as a control. The linker is fused with LamB used as a sandwich-anchoring motif so that the linker can be displayed on the surface of VnDX.

Characterization

1. Identification

After receiving the synthesized DNA, PCR was done to certify that the plasmid was correct, and the experimental results were shown in figure1.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 676
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 824