Coding

Part:BBa_K3722006

Designed by: Yongzhu Li   Group: iGEM21_NWU-CHINA-A   (2021-09-29)
Revision as of 05:22, 21 October 2021 by Liyaa (Talk | contribs) (→‎Biology)


Fre-Linker-SttH

Construction of SttH and Fre fusion enzyme to enhance the solubility and activity of SttH.Fre is a unique N-terminal tag that efficiently enhanced SttH activity by increasing solubility as well as cofactor supply.


Biology

After linked the Fre to SttH, the total expression of SttH decreased, but all the chimeric enzymes showed highly increased ratios of their soluble forms in E. coli. The molar concentrations of soluble Fre-Linker–SttH was 1.64 μM, which was more than three times higher than that of SttH by itself[1].

Usage

References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 961
    Illegal NgoMIV site found at 1219
    Illegal AgeI site found at 1448
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 424


[edit]
Categories
Parameters
None