Plasmid_Backbone

Part:BBa_K176007

Designed by: Hao Jiang, Danqian Liu, Chao Li   Group: iGEM09_USTC   (2009-06-16)
Revision as of 09:05, 16 June 2009 by Horigen (Talk | contribs)

pSB1A3 with the suffix of J61002 (mRFP)

It's the same plasmid backbone of pSB1A3, but sequence between SpeI and PstI in the suffix are from plasmid backbon J61002.

SpeI-RBS-mRFP-Terminator-NotI-PstI-pSB1A3-prefix

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a suffix.
    Illegal SpeI site found at 2
    Illegal PstI site found at 885
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3003
    Illegal SpeI site found at 2
    Illegal PstI site found at 885
    Illegal NotI site found at 878
    Illegal NotI site found at 3009
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3003
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3003
    Plasmid lacks a suffix.
    Illegal SpeI site found at 2
    Illegal PstI site found at 885
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3003
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3018
    Illegal SpeI site found at 2
    Illegal PstI site found at 885
    Illegal AgeI site found at 581
    Illegal AgeI site found at 693
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 2042


[edit]
Categories
Parameters
None