Plasmid_Backbone
azl gibson

Part:BBa_K4074047:Design

Designed by: MIT iGEM 2021   Group: iGEM21_MIT   (2021-10-01)
Revision as of 03:28, 21 October 2021 by Perts (Talk | contribs) (→‎Source)

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pSB_AZL_GIBSON


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 1187
    Illegal suffix found at 3111
    Illegal EcoRI site found at 1961
    Illegal XbaI site found at 1934
    Illegal PstI site found at 1922
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1187
    Illegal EcoRI site found at 1961
    Illegal SpeI site found at 3112
    Illegal PstI site found at 1922
    Illegal PstI site found at 3126
    Illegal NotI site found at 1193
    Illegal NotI site found at 3100
    Illegal NotI site found at 3119
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1187
    Illegal EcoRI site found at 1961
    Illegal BamHI site found at 1940
    Illegal XhoI site found at 171
    Illegal XhoI site found at 1063
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 1187
    Illegal suffix found at 3112
    Illegal EcoRI site found at 1961
    Illegal XbaI site found at 1934
    Illegal PstI site found at 1922
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 1187
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1961
    Illegal XbaI site found at 1202
    Illegal XbaI site found at 1934
    Illegal SpeI site found at 3112
    Illegal PstI site found at 1922
    Illegal PstI site found at 3126
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

-


Source

pSB1C from the iGEM 2021 distribution kit; B. subtilis genome, Erika of the Grossman Lab, Weiss lab

References