Plasmid_Backbone
amyE gibso
Part:BBa_K4074043
Designed by: MIT iGEM 2021 Group: iGEM21_MIT (2021-10-01)
pSB_AMY_GIBSON
Contents: AmyE homology arms (Gibson)
What is it?: A basic part - plasmid backbone
What does it do?: It codes for amyE homology arms, which allow for integration into the amyE locus of the B subtilis chromosome.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 1187
Illegal EcoRI site found at 1772
Illegal EcoRI site found at 2477
Illegal SpeI site found at 3887
Illegal PstI site found at 1960
Illegal PstI site found at 1986
Illegal PstI site found at 2438
Illegal PstI site found at 3901
Illegal NotI site found at 1193
Illegal NotI site found at 3875
Illegal NotI site found at 3894 - 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 1187
Plasmid lacks a suffix.
Illegal EcoRI site found at 1772
Illegal EcoRI site found at 2477
Illegal XbaI site found at 1202
Illegal XbaI site found at 2450
Illegal SpeI site found at 3887
Illegal PstI site found at 1960
Illegal PstI site found at 1986
Illegal PstI site found at 2438
Illegal PstI site found at 3901 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
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Categories
Parameters
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