Part:BBa_K3777013

TetR-T7(tetO)-3WJdB

Basic biosensor device for tetracycline detection.

Biology
The device can be used for tetracycline detection. In presence of erythromycin cells expressing this composite part will produce a fluorescent signal which can be correlated to the amount of compound present in the sample.
Usage

Characterizition
When antibiotics are absent, aTF will bind to the inducible promoter（PI）and prevent RNA polymerase from initiating transcription, thus repressing the expression of reporter gene. If antibiotics are present, aTFs will bind with them and change their conformation. Therefore, they will no longer able to bind to the promoter, resulting in the expression of reporter gene. In our case, fluorescent signal increases only with the presence of antibiotics Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal NheI site found at 1009
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]