Regulatory

Part:BBa_K3783000:Experience

Designed by: Satvik Kethireddy   Group: iGEM21_OhioState   (2021-10-04)
Revision as of 02:39, 21 October 2021 by Rcburrows (Talk | contribs) (→‎OhioState Application)

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Applications of BBa_K3783000

OhioState Application

The promoter was cloned into a luciferase reporter and then transformed into a strain of E.coli expressing the fraR repressor. The luminescence curve for pFraB works as expected. There is a significant decrease, almost half, in expression in fraR- strains compared to fraR+ strains. This shows it is effective in controlling the expression of proteins. The fraR repressor was expressed via the lacZ promoter, therefore the addition of IPTG results in increased repression.

pFraB Graph
Figure 1. pFraB Luciferase Reporter

User Reviews

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