Part:BBa_K3725040:Experience

The T7 Phytophthora Trigger (Part BBa_K3725040) and the Phytophthora Toehold w/ GFP Reporter (Part BBa_K3725010) are intended to be compatible with each other and used in conjunction. To test the compatibility of the trigger sequence with the toehold sequence, a dual-plasmid transformation was performed, and fluorescence was measured in comparison with cells transformed with only the toehold part and pUC19 (positive control). All samples were divided by optical density to obtain a standardized unit. The fluorescence per optical density unit measurement of cells transformed with both the toehold and trigger sequences was greater and had a statistically significant difference in comparison to cells transformed with only the toehold part and pUC19 plasmid.

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Figure 2: This graph compares the mean fluorescence per optical density values of the P. cryptogea toehold switch, positive control (pUC19), and the dual plasmid. The toehold switch and positive control have very low fluorescence per optical density values, whereas the dual plasmid has a higher fluorescence per optical density value, that is significantly different from the other two groups..

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