Coding
albA1

Part:BBa_K3790004

Designed by: Guanqiao Chi   Group: iGEM21_Fudan   (2021-10-01)
Revision as of 01:13, 21 October 2021 by TiTong Fudan (Talk | contribs) (Introduction)


albA1


Introduction

2021 Fudan

Sso7d is a double-stranded binding protein that is linked to DNA polymerase A or DNA polymerase B to produce a fusion protein with higher synthetic efficiency compared to wild-type DNA polymerase.DbpA, a double-stranded binding protein from the same species as Sso7d, Sulfolobus solfataricus, was suggested in previous studies to have a similar function to Sso7d in enhancing DNA polymerase activity. The Bst Pol selected for this experiment was DNA polymerase Ⅰ, and no previous studies have focused on whether double-stranded binding proteins can enhance the activity of DNA polymerase Ⅰ. However, we ventured to guess that the double-stranded binding protein could enhance the related activity of DNA polymerase Ⅰ, and performed the next validation experiments


Usage and Biology

Experimental Results

Reference

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
Parameters
None