Regulatory

Part:BBa_K3788017

Designed by: Rebecca Pagès   Group: iGEM21_Aix-Marseille   (2021-09-28)
Revision as of 21:50, 20 October 2021 by Liam Z (Talk | contribs)


lexA-repressed promoter

Induced by the SOS response (LexA dependent) upon DNA damages (UV, mitomycin…). This promoter allow the expression of the operon caa and cal. caa gene is coding for Colicin A (ColA) a toxin produced by E. coli strains, and active against other non-immune E.coli strains. E. coli have an imumnity protein coded by cai gene (-). cal gene is codding for a lysis protein, when this protein is produce ColA can be delivered in the extracellular medium after the E. coli cells lysis.


Description of the operon

This part is constituted from -35 and -10 boxes, with a LexA box and the beggining of the CDS of caa. The LexA box is a sequence that allows the binding of a tetramere of LexA that represses the trascription of the caa-cal operon.

This repression can be stopped when the SOS response is activated, due to DNA damage that activate RecA production that induce LexA's depolymersation.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None