Coding

Part:BBa_K4006004

Designed by: Margaret Cook   Group: iGEM21_ASU   (2021-10-20)
Revision as of 20:48, 20 October 2021 by Mkcook (Talk | contribs)


MT-6XHis



This part contained a protein coding sequence for metallothionein that has been codon optimized for use in the chloroplast of Chlamydomonas reinhardtii as well as a 6X-histidine tag for visualization of protein expression in a Western blot. Metallothionein is a metal binding protein rich in cysteine that is commonly produced in bacteria and prokaryotes. It has been introduced to the Chlamydomonas genome before and significantly improves the metal binding capacity at low metal concentrations. The polyhistidine tag is well established and has been used previously in C. reinhardtii to tag the photosystem I complex.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 45
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 5
    Illegal SapI.rc site found at 233


[edit]
Categories
Parameters
None