Composite

Part:BBa_K3971028

Designed by: Misaal Bedi   Group: iGEM21_IISER-Pune-India   (2021-10-20)
Revision as of 19:21, 20 October 2021 by Misaalbedi (Talk | contribs)


Cassette to insert sps gene in the Neutral Site 3 of the S. elongatus UTEX 2973 genome.

This composite part has been designed to insert the sps gene into the Neutral Site 3 of the S. elongatus UTEX 2973 genome.

File:T--IISER-Pune-India--spscassette.png
Figure of the composite construct for characterizing cscB transport efficiencies under different promoters. NS1a and NS1b are the two homology arms to Neutral Site 1.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2877
    Illegal PstI site found at 644
    Illegal PstI site found at 2915
    Illegal PstI site found at 3636
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2877
    Illegal NheI site found at 3070
    Illegal PstI site found at 644
    Illegal PstI site found at 2915
    Illegal PstI site found at 3636
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2877
    Illegal BamHI site found at 1196
    Illegal XhoI site found at 2654
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2877
    Illegal PstI site found at 644
    Illegal PstI site found at 2915
    Illegal PstI site found at 3636
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2877
    Illegal PstI site found at 644
    Illegal PstI site found at 2915
    Illegal PstI site found at 3636
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None