DNA

Part:BBa_K3971008

Designed by: Misaal Bedi   Group: iGEM21_IISER-Pune-India   (2021-08-10)
Revision as of 19:15, 20 October 2021 by Misaalbedi (Talk | contribs)

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Portion of the Neutral Site 1 of the Synechococcus elongatus UTEX 2973 genome.

This part is a portion of the Neutral Site 1 of the Synechococcus elongatus UTEX 2973 genome and can be used for homology repair-based modifications in the genome at Neutral Site 1 using the pSL2680 plasmid, which is a Cpf1 based CRISPR plasmid [1].. This was one of the homology arms to Neutral Site 1 in our composite part (BBa_K3971009) which was designed to insert a promoter upstream of a reporter (sYFP2) in order to measure the strength of the promoter, through a Cpf1 CRISPR plasmid. It was also a part of our composite part (BBa_K3971010) which was designed to test the sucrose export efficiencies of the cscB (sucrose permease) transporter under different promoters.

References

1: Cpf1 Is A Versatile Tool for CRISPR Genome Editing Across Diverse Species of Cyanobacteria. Ungerer J, Pakrasi HB. Sci Rep. 2016 Dec 21;6:39681. doi: 10.1038/srep39681. 10.1038/srep39681 PubMed 28000776


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 279
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 92
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 279
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 279
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None