Coding

Part:BBa_K4006001

Designed by: Margaret Cook   Group: iGEM21_ASU   (2021-09-30)
Revision as of 19:10, 20 October 2021 by Mkcook (Talk | contribs)


arsC

This part contained a protein coding sequence for arsenate reductase that has been codon optimized for use in the chloroplast of Chlamydomonas reinhardtii. Arsenate reductase catalyzes the reduction of arsenate [As(V)] to arsenite [As(III)]. Using 2 µM arsenate at a substrate, the Kcat of the protein has been recorded as 4.5 min(-1).

We were able to clone this construct into our plasmid and select for transformed E. coli colonies.

T--ASU--arsCplates.jpg

T--ASU--arsCplates.jpg

Digestion of the miniprepped DNA with SOMETHING and SOMETHING should result in X bands of approximate sizes X and Y.

T--ASU--arsCGel.jpg

Unfortunately, we were unable to successfully transform this construct into C. reinhardtii and test expression or effect on arsenic uptake in the algae.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None