Translational_Unit

Part:BBa_K3765016

Designed by: Dipayan Akhuli   Group: iGEM21_IISc-Bangalore   (2021-10-19)
Revision as of 19:06, 20 October 2021 by Dipayan akhuli (Talk | contribs)

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T7 RBS + SpyCatcher002+dCBD

Translational Unit comprising of the CDS of a SpyCatcher002-dCBD fusion protein under a strong RBS from bacteriophage T7 gene 10.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 667
    Illegal XhoI site found at 309
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 139
    Illegal AgeI site found at 505
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 394


[edit]
Categories
//binding/cellulose
//proteindomain/binding/cellulose
Parameters
None