Measurement

Part:BBa_K3971024

Designed by: Misaal Bedi   Group: iGEM21_IISER-Pune-India   (2021-10-19)
Revision as of 18:57, 20 October 2021 by Misaalbedi (Talk | contribs)


Promoter characterization cassette for native E.coli csc operon bidirectional promoter.

This composite part has been designed to characterize the activity of the native E.coli csc operon bidirectional promoter. In order to allow for modularity in the promoter region of the cassette, two restriction sites Nde1 and BamH1 flank it, so that the promoter can be easily excised out and replaced with another and the cassette can be used to characterize that promoter.

A YFP and a BFP gene flank the promoter on both sides on opposite coding strands, and the relative fluorescence intensity can be used as a proxy for the strength of the promoter in both directions. Both fluorescence promoters have a double terminator (BBa_B0015).

Figure of the composite construct for characterizing the activity of the native E.coli csc operon bidirectional promoter. NS3a and NS3b are the two homology arms to Neutral Site 3.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1699


[edit]
Categories
Parameters
None