Coding

Part:BBa_K3971011:Design

Designed by: Misaal Bedi   Group: iGEM21_IISER-Pune-India   (2021-08-12)
Revision as of 18:51, 20 October 2021 by Misaalbedi (Talk | contribs)

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cscB (sucrose permease) for sucrose transport.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 481
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 525


Design Notes

None.


Source

This gene can be amplified from the pCSCX plasmid, which was a gift from Claudia Vickers (Addgene plasmid # 63918; http://n2t.net/addgene:63918; RRID:Addgene_63918).

References