Coding

Part:BBa_K4006001

Designed by: Margaret Cook   Group: iGEM21_ASU   (2021-09-30)
Revision as of 18:44, 20 October 2021 by Mkcook (Talk | contribs)


arsC

This part contained a protein coding sequence for arsenate reductase that has been codon optimized for use in the chloroplast of Chlamydomonas reinhardtii. Arsenate reductase catalyzes the reduction of arsenate [As(V)] to arsenite [As(III)]. Using 2 µM arsenate at a substrate, the Kcat of the protein has been recorded as 4.5 min(-1).

We were able to clone this construct into our plasmid and select for transformed E. coli colonies.

PICTURE OF PLATES.

Digestion of the miniprepped DNA with X and Y should result in X bands of approximate sizes X and Y.

PICTURE OF GELS.

Unfortunately, we were unable to successfully transform this construct into C. reinhardtii and test expression or effect on arsenic uptake in the algae.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None