Regulatory

Part:BBa_K3861021

Designed by: Mario Delgadillo   Group: iGEM21_Humboldt_Berlin   (2021-10-01)
Revision as of 18:18, 20 October 2021 by Deguevam (Talk | contribs)


araBAD promoter from Salmonella Typhimurium

This is the native promoter of the Salmonella typhimurium araBAD inducer system. PBAD initiates transcription during high L-arabinose and low glucose levels, as PBAD forms a complex with L-arabinose (Guzman et al., 1995).
As a part of our project "TargetTaxi", we aim to use this promoter to regulate the expression of the SPI-1 master regulator, hilA. Thereby, allowing the overexpression of SPI-1 type 3 secretion machinery and its assembly at the cell poles. The presence of the machinery at the cell poles will result in its presence in the membrane of minicells. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None