DNA

Part:BBa_K3971029:Design

Designed by: Misaal Bedi   Group: iGEM21_IISER-Pune-India   (2021-10-20)
Revision as of 18:08, 20 October 2021 by Misaalbedi (Talk | contribs)


gRNA target for Cpf1 based editing in the Neutral Site 3 of Synechococcus elongatus UTEX 2973.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gRNA target sequence was chosen such that it allowed at least 750 bp of the neutral site 3 to be present on either side of the target, and that it did not have any off-target sites in the rest of Synechococcus elongatus UTEX 2973 genome.


Source

This sequence was synthesized.

References