Coding

Part:BBa_K3858013

Designed by: Kun Zhang   Group: iGEM21_GreatBay_United   (2021-10-01)
Revision as of 18:07, 20 October 2021 by Survivor (Talk | contribs)


antiGFP-Low encoding

Recombinant nanobody against GFP. Low affinity compared with antiGFP-High.

Usage and Biology

Reference

1. Fridy P C, Li Y, Keegan S, et al. A robust pipeline for rapid production of versatile nanobody repertoires[J]. Nature methods, 2014, 11(12): 1253-1260.
2. Toda S, McKeithan W L, Hakkinen T J, et al. Engineering synthetic morphogen systems that can program multicellular patterning[J]. Science, 2020, 370(6514): 327-331.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 163
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None