Coding
RFC 37

Part:BBa_K245114:Experience

Designed by: Nika Debeljak   Group: iGEM09_Slovenia   (2009-10-14)
Revision as of 18:03, 20 October 2021 by Ihua li (Talk | contribs)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K245114

User Reviews

UNIQd4ef5e6edad39f70-partinfo-00000000-QINU

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ihua_li NCTU_Formosa(2021)

Cloning Result

We successfully built DenTeeth with LL-37 .

Figure 1. Colony PCR result of DenTeeth after cloning into E. coli BL21. (4685 b.p.)

Cloning Result

Through the modeling built with substituting parameters from published articles, the growth of E. coli and P. gingivalis under the effect of LL-37 would be inhibited significantly. However, the figure also shows that E. coli could still live with LL-37. Simply speaking, we successfully test the feasibility of DenTeeth and find that we can improve the DenTeeth with both biobrick design and efficiency optimization model.

Figure 3. The growth curve of E. coli and P.gingivalis
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BBa_K245114 NCTU-Formosa(2021) Not understood UNIQd4ef5e6edad39f70-partinfo-00000003-QINU