Coding

Part:BBa_K4006003

Designed by: Margaret Cook   Group: iGEM21_ASU   (2021-10-20)
Revision as of 17:56, 20 October 2021 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Ferritin

This part includes a coding sequence for the protein ferritin codon optimized for use in the Chlamydomonas reinhardtii chloroplast. Ferritin is a large protein that takes the form of a hollow sphere, sequestering iron inside of itself. While it is extremely well known as a tool for iron sequestration, it can be used to capture multiple heavy metals. It has also been expressed within the Chlamydomonas reinhardtii chloroplast before with a notable difference in capacity for binding iron. We were able to successfully clone the part into our plasmid and select transformed colonies in E. coli, but we did not obtain any transformant C. reinhardtii colonies after biolistic transformation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 5
    Illegal SapI.rc site found at 515


[edit]
Categories
Parameters
None