Composite

Part:BBa_K3739085

Designed by: Shichen Geng   Group: iGEM21_XMU-China   (2021-09-30)
Revision as of 17:19, 20 October 2021 by Gengshichen (Talk | contribs)


K525998(T7-RBS)-LMT-his-hutH-FT

Histidine ammonia-lyase with a his-tag is involved in catalyzing the reaction of changing L-histidine into trans-urocanate. LMT is a signal peptile. The proteins labeled of tetracysteine motif tag (FlAsH tag, FT in short) can be detected with the biarsenical compound FlAsH.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 257
    Illegal NgoMIV site found at 693
    Illegal NgoMIV site found at 1428
    Illegal NgoMIV site found at 1664
    Illegal AgeI site found at 330
    Illegal AgeI site found at 1157
    Illegal AgeI site found at 1653
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None