Composite

Part:BBa_K3888998:Design

Designed by: Linhan Lei   Group: iGEM21_SJTang   (2021-10-20)
Revision as of 17:12, 20 October 2021 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


UppE Knockout


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 694
    Illegal PstI site found at 1549
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 694
    Illegal PstI site found at 1549
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 694
    Illegal BglII site found at 333
    Illegal XhoI site found at 826
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 694
    Illegal PstI site found at 1549
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 694
    Illegal PstI site found at 1549
    Illegal NgoMIV site found at 38
    Illegal NgoMIV site found at 119
    Illegal NgoMIV site found at 157
    Illegal NgoMIV site found at 831
    Illegal NgoMIV site found at 943
    Illegal NgoMIV site found at 1208
    Illegal NgoMIV site found at 1354
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 538
    Illegal SapI.rc site found at 2037


Design Notes

Flanking region for 1k bp


Source

Rhodopseudomonas palustris CGA009

References