Part:BBa_K3498015:Experience
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UNIQ1c91d1f555c1a169-partinfo-00000000-QINU UNIQ1c91d1f555c1a169-partinfo-00000001-QINU
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IGEM FCB-UANL |
PvanCC promotor + Rsn-2 RBS + Rsn-2 + Terminator This composite part is regulated by vanillic acid sensitive promoter PvanCC. This part contains an histidine tag used for purification. All assembled in pSB3K3 plasmid, a low number of copies plasmid Introduction Ranaspumin-2. Ranaspumin-2, also known as Rsn-2 is a recombinantly produced protein in E. coli Top 10. Its foamability capacity is being tested for fire fighting foam production purposes Results. Protein expression in E. coli Top 10. A characterization of the expression in E. coli was made. For this, different concentrations of inducer (vanillic acid) and different final growing times were tested. Concentrations were determined by imageJ pixel density analyzer program
Foamability The foamability of the expressed Rsn-2 was determined by testing the sonicated cultures, manually agitating a 15 mL corning tube containing 5 mL of the sonicated protein and observing the volume of the foam produced Measurements were taken at 0, 1, 3, 5, 10, 15 and 30 minutes, after that, the measurements were taken every 30 minutes until 12 hours after the agitation Figure 2: Water (negative control), Rsn-2, Rsn-3, Rsn 3-5, SDS (positive control), all agitated 30 second The foamability is obtained by FV/SV where FV is foam volume and SV is solution volume. Ranaspumin-2 made a Foam volume of 4 mL with a 5 mL solution, hence its foamability is of 0.8 (relative number)
Passing the 12 hours the behaviour of the foam was analyzed in order to see how durable it is
It took 8.5 hours for the foam to go from 4 mL to 2 mL (Its half life), and at the final measurement it still had 1 mL of foam |