Coding

Part:BBa_K3739082

Designed by: Shichen Geng   Group: iGEM21_XMU-China   (2021-09-30)
Revision as of 16:33, 20 October 2021 by Gengshichen (Talk | contribs)


Aly01-his-hutH-FT

Histidine ammonia-lyase with a his-tag is involved in catalyzing the reaction of changing L-histidine into trans-urocanate. Aly01 is a signal peptile. The proteins labeled of tetracysteine motif tag (FlAsH tag, FT in short) can be detected with the biarsenical compound FlAsH.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 237
    Illegal NgoMIV site found at 673
    Illegal NgoMIV site found at 1408
    Illegal NgoMIV site found at 1644
    Illegal AgeI site found at 73
    Illegal AgeI site found at 310
    Illegal AgeI site found at 1137
    Illegal AgeI site found at 1633
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None