Coding

Part:BBa_K3739086

Designed by: Shichen Geng   Group: iGEM21_XMU-China   (2021-10-20)
Revision as of 16:25, 20 October 2021 by Registry (Talk | contribs)

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his-hutH-FT

Histidine ammonia-lyase with a his-tag is involved in catalyzing the reaction of changing L-histidine into trans-urocanate. The proteins labeled of tetracysteine motif tag (FlAsH tag, FT in short) can be detected with the biarsenical compound FlAsH.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 162
    Illegal NgoMIV site found at 598
    Illegal NgoMIV site found at 1333
    Illegal NgoMIV site found at 1569
    Illegal AgeI site found at 235
    Illegal AgeI site found at 1062
    Illegal AgeI site found at 1558
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None