Part:BBa_K3896017

Plux_HS-LuxI-EGFP

In the AHL mediated quorum sensing system,after reaching the threshold, AHL can combine with endogenous LuxR to form a complex and activate the corresponding promoter PluxR. In this project, we selected the improved promoter Plux_HS of iGEM2018 Tsinghua.The promoter has the advantage of low leakage.After Plux_ HS is activated, the expressed AHL will combine with endogenous LuxR to form a complex and furtherly activate Plux_ HS promoter, so as to achieve the effect of cascade amplification, and finally express a large number of EGFP, making the detection effect significant.

We first verify the circuits by artificially adding AHL.

We want to know the concentration of AHL and set a series of gradients. The results are as follows.

Fig. The fluorescence intensity of engineered bacteria under different concentrations of AHL.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 721
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1400