Composite

Part:BBa_K3896017

Designed by: Zihan Wu   Group: iGEM21_NEU_CHINA   (2021-10-06)
Revision as of 16:20, 20 October 2021 by Wuzihan (Talk | contribs)


Plux_HS-LuxI-EGFP

In the AHL mediated quorum sensing system,after reaching the threshold, AHL can combine with endogenous LuxR to form a complex and activate the corresponding promoter PluxR. In this project, we selected the improved promoter Plux_HS of iGEM2018 Tsinghua.The promoter has the advantage of low leakage.After Plux_ HS is activated, the expressed AHL will combine with endogenous LuxR to form a complex and furtherly activate Plux_ HS promoter, so as to achieve the effect of cascade amplification, and finally express a large number of EGFP, making the detection effect significant.

We first verify the circuits by artificially adding AHL.

We want to know the concentration of AHL and set a series of gradients. The results are as follows.

T--NEU_CHINA--AHL_suoxiaoban.png

Fig. tuzhu

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 721
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1400


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