Part:BBa_K592015:Design
amilCP Blue Coding Device
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We found this design very useful when assembling components that contain RFP, mCherry, etc. The assembled parts turn red and are not recognizable from the background insert(BBa_J04450) if all colonies becomes red after transformation. Luckily, very few genes produce things that turn blue/purple, which makes our construct extremely useful.
B0014 instead of B0015 in order to avoid unwanted primer binding, etc. J23101 promoter to ensure strong and constitutive expression at all times.
BBa_K592015 is a amilCP blue coding device, which consists of J23101, B0034, K592009, and B0014. It was constructed by iGEM11_Uppsala-Sweden in Sep. 2011. This device can express blue/purple color protein, which enriches the reporter genes for the research of life science. K592009 is a blue chromo protein gene from the Acropora millepora. AmilCP, naturally exhibits strong color when expressed. The protein has an absorbance maximum at 588 nm giving it a blue/purple color visible to the naked eye, thereby requiring no instruments to observe. The strong color is readily observed in both LB or agar culture, in less than 24 hours of incubation. It is a very useful part.
Source
amilCP comes from the genome of Acropora millepora. All other parts are assembled from the iGEM 2011 distribution kit.