RNA

Part:BBa_K3815011

Designed by: Hiroto Koga   Group: iGEM21_Kyoto   (2021-10-18)
Revision as of 16:00, 20 October 2021 by Koga (Talk | contribs)


Part of the V-ATPaseB gene of Frankliniella occidentalis to synthesize dsRNA for RNAi ATPaseB gene of Frankliniella occidentalis to synthesize dsRNA for RNAi. To product dsRNA this sequence inserted in L4440 plasmid, and transformed into HT115(DE3).

purpose

We focused on feeding RNAi to kill these insects, based on previous studies showing that oral ingestion of dsRNA can induce RNAi-induced gene knockdown in Frankliniella occidentalis.
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 452
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 320


production and purification methods



[edit]
Categories
Parameters
None