Composite

Part:BBa_K3888999:Design

Designed by: Xiaoxuan Cheng   Group: iGEM21_SJTang   (2021-10-20)
Revision as of 15:27, 20 October 2021 by Registry (Talk | contribs)

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HupS knockout


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1521
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1521
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1521
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1521
    Illegal NgoMIV site found at 121
    Illegal NgoMIV site found at 258
    Illegal NgoMIV site found at 690
    Illegal NgoMIV site found at 1068
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 414


Design Notes

Our flanking region is about 1kbp in length for deletion.


Source

the genome of R. Palustris

References