Composite

Part:BBa_K3888999

Designed by: Xiaoxuan Cheng   Group: iGEM21_SJTang   (2021-10-20)
Revision as of 15:27, 20 October 2021 by Registry (Talk | contribs)

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HupS knockout

Uptake hydrogenase is a hydrogenase that recycles hydrogen produced by nitrogenase and converts it back into protons, whose expression reduces hydrogen production. The uptake hydrogenase is composed of small and large subunits, encoded by hupS and hupL, respectively. HupS mediates electron transport from the active site of the large subunit to redox partners and downstream reactions through a set of FeS clusters. HupL harbors the active site that contains the metals Ni and Fe.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1521
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1521
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1521
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1521
    Illegal NgoMIV site found at 121
    Illegal NgoMIV site found at 258
    Illegal NgoMIV site found at 690
    Illegal NgoMIV site found at 1068
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 414


[edit]
Categories
Parameters
None