Part:BBa_K1819001

Latex Clearing Protein (Lcp) - rubber degradation

Latex clearing protein (Lcp) from actinomycete Streptomyces sp. strain K30 (GenBank accession code: AAR25849.1) is a 42kDa enzyme which catalyzes rubber cleavage at the cis double bonds to multiple products ranging from C20 tetra-isoprenoid to at least C35 hepta-isoprenoid with measured specific activity of 1.3 mol/min per mg.

It contains an oxidized heme-Fe³⁺ not bound to dioxygen.

Figure 1 - Schematic representation of BBa_K1819001 insert

figure 2 - Gel analysis confirmation of Lcp in pSB1C3

We performed a bioinformatics analysis of Lcp predicting its structure, creating an HMM model and comparing RoxA and Lcp secondary structure based on reported data. Click here to see our analysis

Please note this part was submitted in pSB1C3, the Registry's standard shipping backbone, according to submission requirements. However, pSB1C3 contains a SacI site and if you want to use this part we recommend to move the part into another plasmid backbone what can be easily done.

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Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 889
  Illegal XhoI site found at 1043
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 535
  Illegal NgoMIV site found at 857
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 438