Part:BBa_K3815015

AANDENYALGA. mutant SsrA degradation tag

Usage and Biology

This is an engineered derivative of wildtype ssrA tag from Escherichia coli, where three C-terminal amino acids LAA in WT are replaced to LGA. To quantify its efficiency of protein degradation, GFP fluorescence intensity of E.coli overnight culture bearing a plasmid expressing GFP-mutant ssrA tag fusion protein was measured by Qubit, and then compared to that of WT and other mutants. Refer to BBa_M0050 for the biological function of the tag.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Result

obtained by mutagenizing the WT tag with mixed primers.

Improvement from existing parts

This is a part improved from BBa_K1051206. In this part, three C-terminal amino acids LAA were replaced with LGA, resulting in a reduced protein degradation efficiency.