Coding

Part:BBa_K3735017

Designed by: Jiandong Jiang   Group: iGEM21_NAU-CHINA   (2021-10-01)
Revision as of 14:54, 20 October 2021 by YihengYang (Talk | contribs)


Coding sequence

In order to improve the quality of SeNPs and improve the reduction efficiency, we hope to link those standard parts and then, two genes express at the same time. This part consist of low intensity SefA and low intensity ssuE. After verifying the function of SefA and ssuE separately, we integrate them and obtain nine combinations of different promoter strengths. And the low SefA and the low ssuE Can produce the best SeNPs.

In order to explore the optimal conditions for the production of SeNPs,we used the Response surface model.We hope to maximize SeNPs production by optimizing temperature, pH and selenite concentration. First, we conducted a preliminary experiment on these three factors, and we chose the best three gradients(Fig.1) of these three factors to do further experiments. 

                   <img src='https://2021.igem.org/wiki/images/c/c7/T--NAU-CHINA--zuhe.png'>

Fig.1 Fig.1 Preliminary experiment results of response surface experiment. A: pH; B: concentration of selenite; C: Temperature. The results of preliminary experiment show that the SeNPs yield is the best when pH are 6,7,8 temperature are 16℃,30℃,37℃ and selenite concentration are 12mM,15mM,18mM.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 3099
    Illegal NheI site found at 3122
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1537
    Illegal BglII site found at 2854
    Illegal BglII site found at 3288
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 329
    Illegal NgoMIV site found at 644
    Illegal NgoMIV site found at 770
    Illegal NgoMIV site found at 1151
    Illegal NgoMIV site found at 1574
    Illegal NgoMIV site found at 2675
    Illegal NgoMIV site found at 2924
    Illegal AgeI site found at 671
    Illegal AgeI site found at 1217
    Illegal AgeI site found at 1373
    Illegal AgeI site found at 1514
    Illegal AgeI site found at 1937
    Illegal AgeI site found at 2294
    Illegal AgeI site found at 2465
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None