Protein_Domain
Part:BBa_K3829005
Designed by: Jing Zhou Group: iGEM21_IvyMaker-China (2021-10-20)
Signal peptide-SS
The signal peptide (SS) on the N terminus can lead the protein out of the cell.
Characterization
In our experiment, signal peptide (SS) was used. SS was a short peptide that could secrete proteins outside the cell. We constructed different gene circuits as shown in Figure 1 to verify the function of SS. The results showed that When SS was not present, fluorescence diffused in the cell (positive control). When SS was present (4609 and 5105), the fluorescence was displayed on the cell membrane, indicating that SS could secrete proteins outside the cell.
<img src="">
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
//awards/basic_part/nominee
None |