Coding

Part:BBa_K3888005:Experience

Designed by: Linhan Lei   Group: iGEM21_SJTang   (2021-10-01)
Revision as of 14:32, 20 October 2021 by RyanLei (Talk | contribs)


Applications of BBa_K3888005

In order to achieve gene knockout, we used pK18mobsacB-Gm plasmid as a knockout vector. In addition, in order to achieve knockout, we selected about 1000bp sequences in the upstream and downstream of UppE and HupS respectively for design, amplified from the Rhodopseudomonas palustris CGA009 by PCR, and constructed knockout plasmids by the Gibson method (see the Design section for principles) ). The results of plasmid design and construction are as follows: UppE experiments.png

User Reviews

UNIQ1f8237afb8605219-partinfo-00000000-QINU UNIQ1f8237afb8605219-partinfo-00000001-QINU