Regulatory

Part:BBa_K3726076:Design

Designed by: Santiago Barragán Ariza   Group: iGEM21_MADRID_UCM   (2021-10-08)
Revision as of 14:14, 20 October 2021 by Sbarraga (Talk | contribs) (→‎Design Notes)

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P_pBAD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

The part is a phytobrick compatible MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. As a promoter part, the upstream overhang is GGAG, while the downstream overhang is TACTAGAG , where AGAG are extra bases for optimal ribosomal binding, in accordance with the Marburg Collection design guidelines.

The Sapl site should be removed in order to make this part RFC1000 compatible. however, this part is MoClo compatible with the marburg collection.

Source

References

N. Lee, W. Gielow and R. Wallace, "Mechanism of araC autoregulation and the domains of two overlapping promoters, Pc and PBAD, in the L-arabinose regulatory region of Escherichia coli.", 2021.