Composite
Part:BBa_K3726059:Design
Designed by: Santiago Barragán Ariza Group: iGEM21_MADRID_UCM (2021-10-20)
L2_BOH2A-FRRRF
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1230
Illegal EcoRI site found at 1873
Illegal PstI site found at 18
Illegal PstI site found at 1778
Illegal PstI site found at 2026
Illegal PstI site found at 3327
Illegal PstI site found at 6974 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1230
Illegal EcoRI site found at 1873
Illegal NheI site found at 5654
Illegal NheI site found at 5677
Illegal NheI site found at 5980
Illegal NheI site found at 6208
Illegal PstI site found at 18
Illegal PstI site found at 1778
Illegal PstI site found at 2026
Illegal PstI site found at 3327
Illegal PstI site found at 6974 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1230
Illegal EcoRI site found at 1873
Illegal BglII site found at 7369
Illegal BglII site found at 7486
Illegal BglII site found at 9905
Illegal BamHI site found at 3410
Illegal BamHI site found at 3749
Illegal XhoI site found at 4682
Illegal XhoI site found at 8589
Illegal XhoI site found at 9432 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1230
Illegal EcoRI site found at 1873
Illegal PstI site found at 18
Illegal PstI site found at 1778
Illegal PstI site found at 2026
Illegal PstI site found at 3327
Illegal PstI site found at 6974 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1230
Illegal EcoRI site found at 1873
Illegal PstI site found at 18
Illegal PstI site found at 1778
Illegal PstI site found at 2026
Illegal PstI site found at 3327
Illegal PstI site found at 6974
Illegal NgoMIV site found at 1517
Illegal NgoMIV site found at 1617
Illegal NgoMIV site found at 1923
Illegal NgoMIV site found at 4005
Illegal AgeI site found at 594
Illegal AgeI site found at 2295
Illegal AgeI site found at 3533 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
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Source
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