Coding

Part:BBa_K3747000:Design

Designed by: Riemer van der Vliet   Group: iGEM21_Wageningen_UR   (2021-10-20)
Revision as of 13:02, 20 October 2021 by Riemer (Talk | contribs)

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pMMO-B1. Particulate Methane Monooxygeanse substructure B1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 19
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 19
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 19
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 19
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 19
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 325


Design Notes

Substructure B1


Source

Methylococcus capsulatus Bath

References

Kim, H.J., Huh, J., Kwon, Y.W. et al. Biological conversion of methane to methanol through genetic reassembly of native catalytic domains. Nat Catal 2, 342–353 (2019). https://doi.org/10.1038/s41929-019-0255-1