RNA
Part:BBa_K3752000
Designed by: Tudor Onose Group: iGEM21_Warwick (2021-10-20)
Revision as of 12:49, 20 October 2021 by PaintMeTed (Talk | contribs)
OXA-48 Parent cgRNA
cgRNA which senses the mRNA of OXA-48 - used as a riboswitch by 2021 Warwick iGEM.
Sequence and Features
NUPACK modelling was used to confirm the function of this part in silico as laboratory work could not be used to verify this due to time constraints.
As seen above, the natively folded state of the cgRNA does not present the dCas9 binding handle.
Yet when the cgRNA anneals with the OXA-48 mRNA, the dCas9 binding handle (top in this image) is folded correctly, switching the CRISPRa system on.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |